Use of oligonucleotides to generate large deletions
نویسندگان
چکیده
منابع مشابه
A simple strategy to generate small deletions using Bal31 nuclease.
The definition of the functional boundaries of regulatory DNA elements often includes sequential deletion of DNA using controlled digestion with BaBl nuclease [for example, (1, 2)]. To obtain small deletions (< 100 bp) we have devised a simple strategy involving cloning of the target DNA into the EcoSl site of the pBR322 plasmid. The hybrid plasmid is linearized at a unique restriction site pre...
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Spontaneous chromosomal breakages frequently occur at genomic hot spots in the absence of DNA damage and can result in translocation-related human disease. Chromosomal breakpoints are often mapped near purine-pyrimidine Z-DNA-forming sequences in human tumors. However, it is not known whether Z-DNA plays a role in the generation of these chromosomal breakages. Here, we show that Z-DNA-forming s...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1986
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/14.12.5115